Identification of three elicitins and a galactan-based complex polysaccharide from a concentrated culture filtrate of Phytophthora infestans efficient against Pectobacterium atrosepticum.
Identifieur interne : 001070 ( Main/Exploration ); précédent : 001069; suivant : 001071Identification of three elicitins and a galactan-based complex polysaccharide from a concentrated culture filtrate of Phytophthora infestans efficient against Pectobacterium atrosepticum.
Auteurs : Guillaume Saubeau [France] ; Fanny Gaillard [France] ; Laurent Legentil [France] ; Caroline Nugier-Chauvin [France] ; Vincent Ferrières [France] ; Didier Andrivon [France] ; Florence Val [France]Source :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2014.
Descripteurs français
- KwdFr :
- Activation enzymatique (effets des médicaments et des substances chimiques), Alignement de séquences (MeSH), Antibactériens (composition chimique), Antibactériens (pharmacologie), Données de séquences moléculaires (MeSH), Extraits de plantes (composition chimique), Extraits de plantes (pharmacologie), Fractionnement chimique (MeSH), Pectobacterium (effets des médicaments et des substances chimiques), Phenylalanine ammonia-lyase (métabolisme), Phytophthora infestans (métabolisme), Polyosides (composition chimique), Polyosides (pharmacologie), Protéines (composition chimique), Protéines (pharmacologie), Solanum tuberosum (effets des médicaments et des substances chimiques), Solanum tuberosum (enzymologie), Spectrométrie de masse MALDI (MeSH), Spectrométrie de masse en tandem (MeSH), Séquence d'acides aminés (MeSH).
- MESH :
- composition chimique : Antibactériens, Extraits de plantes, Polyosides, Protéines.
- effets des médicaments et des substances chimiques : Activation enzymatique, Pectobacterium, Solanum tuberosum.
- enzymologie : Solanum tuberosum.
- métabolisme : Phenylalanine ammonia-lyase, Phytophthora infestans.
- pharmacologie : Antibactériens, Extraits de plantes, Polyosides, Protéines.
- Alignement de séquences, Données de séquences moléculaires, Fractionnement chimique, Spectrométrie de masse MALDI, Spectrométrie de masse en tandem, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Anti-Bacterial Agents (chemistry), Anti-Bacterial Agents (pharmacology), Chemical Fractionation (MeSH), Enzyme Activation (drug effects), Molecular Sequence Data (MeSH), Pectobacterium (drug effects), Phenylalanine Ammonia-Lyase (metabolism), Phytophthora infestans (metabolism), Plant Extracts (chemistry), Plant Extracts (pharmacology), Polysaccharides (chemistry), Polysaccharides (pharmacology), Proteins (chemistry), Proteins (pharmacology), Sequence Alignment (MeSH), Solanum tuberosum (drug effects), Solanum tuberosum (enzymology), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Tandem Mass Spectrometry (MeSH).
- MESH :
- chemical , chemistry : Anti-Bacterial Agents, Plant Extracts, Polysaccharides, Proteins.
- chemical , metabolism : Phenylalanine Ammonia-Lyase.
- chemical , pharmacology : Anti-Bacterial Agents, Plant Extracts, Polysaccharides, Proteins.
- drug effects : Enzyme Activation, Pectobacterium, Solanum tuberosum.
- enzymology : Solanum tuberosum.
- metabolism : Phytophthora infestans.
- Amino Acid Sequence, Chemical Fractionation, Molecular Sequence Data, Sequence Alignment, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry.
Abstract
The induction of plant immunity by Pathogen Associated Molecular Patterns (PAMPs) constitutes a powerful strategy for crop protection. PAMPs indeed induce general defense responses in plants and thus increase plant resistance to pathogens. Phytophthora infestans culture filtrates (CCFs) are known to induce defense responses and decrease the severity of soft rot due to Pectobacterium atrosepticum in potato tubers. The aim of this study was to identify and characterize the active compounds from P. infestans filtrate. The filtrate was fractionated by gel filtration, and the protection effects against P. atrosepticum and the ability to induce PAL activity were tested for each fraction. The fraction active in protection (F1) also induced PAL activity, as did the whole filtrate. Three elicitins (INF1, INF4 and INF5) were identified in F1b, subfraction of F1, by MALDI-TOF-MS and MS/MS analyses. However, deproteinized F1b still showed biological activity against the bacterium, revealing the presence of an additional active compound. GC-MS analyses of the deproteinized fraction highlighted the presence of a galactan-based complex polysaccharide. These experiments demonstrate that the biological activity of the CCF against P. atrosepticum results from a combined action of three elicitins and a complex polysaccharide, probably through the activation of general defense responses.
DOI: 10.3390/molecules191015374
PubMed: 25264828
PubMed Central: PMC6270706
Affiliations:
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wicri:level="3"><nlm:affiliation>CNRS-Université Pierre et Marie Curie, FR2424, Station Biologique de Roscoff, Roscoff-Cedex 29682, France. gaillard@sb-roscoff.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>CNRS-Université Pierre et Marie Curie, FR2424, Station Biologique de Roscoff, Roscoff-Cedex 29682</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Legentil, Laurent" sort="Legentil, Laurent" uniqKey="Legentil L" first="Laurent" last="Legentil">Laurent Legentil</name><affiliation wicri:level="3"><nlm:affiliation>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France. laurent.legentil@ensc-rennes.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Nugier Chauvin, Caroline" sort="Nugier Chauvin, Caroline" uniqKey="Nugier Chauvin C" first="Caroline" last="Nugier-Chauvin">Caroline Nugier-Chauvin</name><affiliation wicri:level="3"><nlm:affiliation>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France. florence.val@agrocampus-ouest.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Ferrieres, Vincent" sort="Ferrieres, Vincent" uniqKey="Ferrieres V" first="Vincent" last="Ferrières">Vincent Ferrières</name><affiliation wicri:level="3"><nlm:affiliation>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France. vincent.ferrieres@ensc-rennes.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Andrivon, Didier" sort="Andrivon, Didier" uniqKey="Andrivon D" first="Didier" last="Andrivon">Didier Andrivon</name><affiliation wicri:level="3"><nlm:affiliation>INRA, UMR1349 IGEPP, Le Rheu-Cedex F-35653, France. didier.andrivon@rennes.inra.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, UMR1349 IGEPP, Le Rheu-Cedex F-35653</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author><author><name sortKey="Val, Florence" sort="Val, Florence" uniqKey="Val F" first="Florence" last="Val">Florence Val</name><affiliation wicri:level="3"><nlm:affiliation>Agrocampus Ouest, UMR1349 IGEPP, Rennes F-35000, France. florence.val@agrocampus-ouest.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Agrocampus Ouest, UMR1349 IGEPP, Rennes F-35000</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region></placeName></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Anti-Bacterial Agents (chemistry)</term><term>Anti-Bacterial Agents (pharmacology)</term><term>Chemical Fractionation (MeSH)</term><term>Enzyme Activation (drug effects)</term><term>Molecular Sequence Data (MeSH)</term><term>Pectobacterium (drug effects)</term><term>Phenylalanine Ammonia-Lyase (metabolism)</term><term>Phytophthora infestans (metabolism)</term><term>Plant Extracts (chemistry)</term><term>Plant Extracts (pharmacology)</term><term>Polysaccharides (chemistry)</term><term>Polysaccharides (pharmacology)</term><term>Proteins (chemistry)</term><term>Proteins (pharmacology)</term><term>Sequence Alignment (MeSH)</term><term>Solanum tuberosum (drug effects)</term><term>Solanum tuberosum (enzymology)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique (effets des médicaments et des substances chimiques)</term><term>Alignement de séquences (MeSH)</term><term>Antibactériens (composition chimique)</term><term>Antibactériens (pharmacologie)</term><term>Données de séquences moléculaires (MeSH)</term><term>Extraits de plantes (composition chimique)</term><term>Extraits de plantes (pharmacologie)</term><term>Fractionnement chimique (MeSH)</term><term>Pectobacterium (effets des médicaments et des substances chimiques)</term><term>Phenylalanine ammonia-lyase (métabolisme)</term><term>Phytophthora infestans (métabolisme)</term><term>Polyosides (composition chimique)</term><term>Polyosides (pharmacologie)</term><term>Protéines (composition chimique)</term><term>Protéines (pharmacologie)</term><term>Solanum tuberosum (effets des médicaments et des substances chimiques)</term><term>Solanum tuberosum (enzymologie)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Plant Extracts</term><term>Polysaccharides</term><term>Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phenylalanine Ammonia-Lyase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Plant Extracts</term><term>Polysaccharides</term><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Antibactériens</term><term>Extraits de plantes</term><term>Polyosides</term><term>Protéines</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Enzyme Activation</term><term>Pectobacterium</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Activation enzymatique</term><term>Pectobacterium</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phenylalanine ammonia-lyase</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antibactériens</term><term>Extraits de plantes</term><term>Polyosides</term><term>Protéines</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Chemical Fractionation</term><term>Molecular Sequence Data</term><term>Sequence Alignment</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Données de séquences moléculaires</term><term>Fractionnement chimique</term><term>Spectrométrie de masse MALDI</term><term>Spectrométrie de masse en tandem</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The induction of plant immunity by Pathogen Associated Molecular Patterns (PAMPs) constitutes a powerful strategy for crop protection. PAMPs indeed induce general defense responses in plants and thus increase plant resistance to pathogens. Phytophthora infestans culture filtrates (CCFs) are known to induce defense responses and decrease the severity of soft rot due to Pectobacterium atrosepticum in potato tubers. The aim of this study was to identify and characterize the active compounds from P. infestans filtrate. The filtrate was fractionated by gel filtration, and the protection effects against P. atrosepticum and the ability to induce PAL activity were tested for each fraction. The fraction active in protection (F1) also induced PAL activity, as did the whole filtrate. Three elicitins (INF1, INF4 and INF5) were identified in F1b, subfraction of F1, by MALDI-TOF-MS and MS/MS analyses. However, deproteinized F1b still showed biological activity against the bacterium, revealing the presence of an additional active compound. GC-MS analyses of the deproteinized fraction highlighted the presence of a galactan-based complex polysaccharide. These experiments demonstrate that the biological activity of the CCF against P. atrosepticum results from a combined action of three elicitins and a complex polysaccharide, probably through the activation of general defense responses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25264828</PMID><DateCompleted><Year>2015</Year><Month>06</Month><Day>10</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>10</Issue><PubDate><Year>2014</Year><Month>Sep</Month><Day>26</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>Identification of three elicitins and a galactan-based complex polysaccharide from a concentrated culture filtrate of Phytophthora infestans efficient against Pectobacterium atrosepticum.</ArticleTitle><Pagination><MedlinePgn>15374-90</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules191015374</ELocationID><Abstract><AbstractText>The induction of plant immunity by Pathogen Associated Molecular Patterns (PAMPs) constitutes a powerful strategy for crop protection. PAMPs indeed induce general defense responses in plants and thus increase plant resistance to pathogens. Phytophthora infestans culture filtrates (CCFs) are known to induce defense responses and decrease the severity of soft rot due to Pectobacterium atrosepticum in potato tubers. The aim of this study was to identify and characterize the active compounds from P. infestans filtrate. The filtrate was fractionated by gel filtration, and the protection effects against P. atrosepticum and the ability to induce PAL activity were tested for each fraction. The fraction active in protection (F1) also induced PAL activity, as did the whole filtrate. Three elicitins (INF1, INF4 and INF5) were identified in F1b, subfraction of F1, by MALDI-TOF-MS and MS/MS analyses. However, deproteinized F1b still showed biological activity against the bacterium, revealing the presence of an additional active compound. GC-MS analyses of the deproteinized fraction highlighted the presence of a galactan-based complex polysaccharide. These experiments demonstrate that the biological activity of the CCF against P. atrosepticum results from a combined action of three elicitins and a complex polysaccharide, probably through the activation of general defense responses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Saubeau</LastName><ForeName>Guillaume</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>INRA, UMR1349 IGEPP, Le Rheu-Cedex F-35653, France. guillaume.saubeau@agrocampus-ouest.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gaillard</LastName><ForeName>Fanny</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>CNRS-Université Pierre et Marie Curie, FR2424, Station Biologique de Roscoff, Roscoff-Cedex 29682, France. gaillard@sb-roscoff.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Legentil</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France. laurent.legentil@ensc-rennes.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nugier-Chauvin</LastName><ForeName>Caroline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France. florence.val@agrocampus-ouest.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ferrières</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Ecole Nationale Supérieure de Chimie de Rennes, CNRS, UMR 6226, 11 Allée de Beaulieu, CS 50837, Rennes Cedex 7 35708, France. vincent.ferrieres@ensc-rennes.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andrivon</LastName><ForeName>Didier</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>INRA, UMR1349 IGEPP, Le Rheu-Cedex F-35653, France. didier.andrivon@rennes.inra.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Val</LastName><ForeName>Florence</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Agrocampus Ouest, UMR1349 IGEPP, Rennes F-35000, France. florence.val@agrocampus-ouest.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>09</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C085101">elicitin, Phytophthora</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.3.1.24</RegistryNumber><NameOfSubstance UI="D010650">Phenylalanine Ammonia-Lyase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000737" 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PubStatus="pubmed"><Year>2014</Year><Month>9</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>6</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25264828</ArticleId><ArticleId IdType="pii">molecules191015374</ArticleId><ArticleId IdType="doi">10.3390/molecules191015374</ArticleId><ArticleId IdType="pmc">PMC6270706</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Fungal Genet Biol. 1999 Nov;28(2):94-106</Citation><ArticleIdList><ArticleId IdType="pubmed">10587472</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2000 Nov;124(3):1027-38</Citation><ArticleIdList><ArticleId IdType="pubmed">11080280</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 1999 Dec;56(11-12):1020-47</Citation><ArticleIdList><ArticleId 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first="Guillaume" last="Saubeau">Guillaume Saubeau</name></region><name sortKey="Andrivon, Didier" sort="Andrivon, Didier" uniqKey="Andrivon D" first="Didier" last="Andrivon">Didier Andrivon</name><name sortKey="Ferrieres, Vincent" sort="Ferrieres, Vincent" uniqKey="Ferrieres V" first="Vincent" last="Ferrières">Vincent Ferrières</name><name sortKey="Gaillard, Fanny" sort="Gaillard, Fanny" uniqKey="Gaillard F" first="Fanny" last="Gaillard">Fanny Gaillard</name><name sortKey="Legentil, Laurent" sort="Legentil, Laurent" uniqKey="Legentil L" first="Laurent" last="Legentil">Laurent Legentil</name><name sortKey="Nugier Chauvin, Caroline" sort="Nugier Chauvin, Caroline" uniqKey="Nugier Chauvin C" first="Caroline" last="Nugier-Chauvin">Caroline Nugier-Chauvin</name><name sortKey="Val, Florence" sort="Val, Florence" uniqKey="Val F" first="Florence" last="Val">Florence Val</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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